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Technologies for extracting lipids from oleaginous microorganisms for biodiesel production

Cunwen WANG, Lu CHEN, Bajpai RAKESH, Yuanhang QIN, Renliang LV

《能源前沿(英文)》 2012年 第6卷 第3期   页码 266-274 doi: 10.1007/s11708-012-0193-y

摘要: Recently, biodiesel has received much more attention. Soybean oil, rapeseed oil, palm oil and corn oil are primary feedstock for biodiesel production. However, biodiesel production from these traditional oil-rich crops is limited by land availability, climate, and environmental and social issues regarding the use of feed and food crops for fuel. Oleaginous microorganisms, including microalgae, bacteria, yeast and fungi can be cultivated with high lipid contents and used as promising feedstock for biodiesel production. However, the high cost of biodiesel production using oil microorganisms has been the biggest obstacle for its industrialization. The process of biodiesel production from microorganisms involves many steps, of which the lipids extraction is the most important and costly. Therefore, searching for an effective and economical extraction system is critical. Various approaches of lipids extraction are discussed in this review, including traditional extraction procedures such as solvent extraction, pressing and solvent integrated extraction, as well as some new procedures.

关键词: oleaginous microorganisms     biodiesel     solvent extraction     ultrasound-assisted extraction     microwave-assisted extraction     supercritical fluid extraction (SFE)    

Response of soil microorganisms to vegetational succession in Ziwuling Forest

Hong ZHANG, Jialong LU, Shiwei ZHAO,

《环境科学与工程前沿(英文)》 2009年 第3卷 第4期   页码 421-427 doi: 10.1007/s11783-009-0147-8

摘要: This study investigates the changes in soil microbial populations as vegetational succession progress from abandoned farmland to climax forest in the Ziwuling Forest, which is located in the northern part of the Loess Plateau, China. Different organic C and total extractable N between the fumigated and non-fumigated soils were assumed to be released from soil microorganisms. Soil microbial C was calculated using ec=0.38 and microbial N was calculated using en=0.45. The released P was converted to microbial biomass P using ep=0.40. Soil bacteria, fungi, and actinomycetes increased as the vegetational succession progressed. Microbial C was of the highest amount in farmland. Microbial C, N, and P generally increased from abandoned land to climax community. The results indicated significant (<0.05) diversities of soil microbial biomass under different vegetation. There was a significant correlation between microbial biomass and soil nutrients. Knowledge about soil microbial populations is important for forecasting vegetational succession and determining the ecological condition of the environment.

关键词: soil microorganisms     vegetational succession     Ziwuling Forest    

Degradation of bisphenol A by microorganisms immobilized on polyvinyl alcohol microspheres

Xue BAI, Hanchang SHI, Zhengfang YE, Qiujin SUN, Qing WANG, Zhongyou WANG

《环境科学与工程前沿(英文)》 2013年 第7卷 第6期   页码 844-850 doi: 10.1007/s11783-013-0487-2

摘要: In this study, microorganisms (named B111) were immobilized on polyvinyl alcohol microspheres prepared by the inverse suspension crosslinked method. The biodegradation of bisphenol A (BPA) and 4-hydroxybenzaldehyde, a degradation product of BPA, by free and immobilized B111 was investigated. The BPA degradation studies were carried out at initial BPA concentrations ranging from 25 to 150 mg·L . The affinity constant K and maximum degradation rate were 98.3 mg·L and 19.7 mg·mg VSS·d for free B111, as well as 87.2 mg·L and 21.1 mg·mg VSS·d for immobilized B111, respectively. 16S rDNA gene sequence analyses confirmed that the dominant genera were and for BPA biodegradation in microorganisms B111.

关键词: bisphenol A     polyvinyl alcohol     immobilized microorganisms     4-hydroxybenzaldehyde    

Advances in airborne microorganisms detection using biosensors: A critical review

《环境科学与工程前沿(英文)》 2021年 第15卷 第3期 doi: 10.1007/s11783-021-1420-8

摘要:

Humanity has been facing the threat of a variety of infectious diseases. Airborne microorganisms can cause airborne infectious diseases, which spread rapidly and extensively, causing huge losses to human society on a global scale. In recent years, the detection technology for airborne microorganisms has developed rapidly; it can be roughly divided into biochemical, immune, and molecular technologies.

关键词: Biosensor     Airborne microorganisms     Microbiological detection technology    

Formation of disinfection byproducts from accumulated soluble products of oleaginous microalga after

Yu Liu, Qiao Zhang, Yu Hong

《环境科学与工程前沿(英文)》 2017年 第11卷 第6期 doi: 10.1007/s11783-017-0938-2

摘要: When microalgae are simultaneously applied for wastewater treatment and lipid production, soluble algal products (SAP) should be paid much attention, as they are important precursors for formation of disinfection byproducts (DBPs), which have potential risks for human health. sp. HQ is an oleaginous microalga that can generate SAP during growth, especially in the exponential phase. This study investigated the contribution of SAP from sp. HQ to DBP formation after chlorination. The predominant DBP precursors from SAP were identified with the 3D excitation-emission matrix fluorescence. After chlorination, a significant reduction was observed in the fluorescence intensity of five specific fluorescence regions, particularly aromatic proteins and soluble microbial by-product-like regions, accompanied with slight shifting of the peak. The produced DBPs were demonstrated to include trihalomethanes and haloacetic acids. As the algal cultivation time was extended in wastewater, the accumulated SAP strengthened the formation of DBPs. The trend for DBP formation was as follows: chloroform>dichloroacetic acid>trichloroacetic acid.

关键词: Chlorella sp. HQ     Chlorination     Disinfection byproducts     Fluorescence spectroscopy     Soluble algal products    

Inactivation and risk control of pathogenic microorganisms in municipal sludge treatment: A review

《环境科学与工程前沿(英文)》 2022年 第16卷 第6期 doi: 10.1007/s11783-021-1504-5

摘要:

• Diversity and detection methods of pathogenic microorganisms in sludge.

关键词: Sludge treatment     Pathogenic microorganisms     Inactivation mechanisms     Exposure risks     Land application    

Determination of growth kinetics of microorganisms linked with 1,4-dioxane degradation in a consortium

《环境科学与工程前沿(英文)》 2022年 第16卷 第5期 doi: 10.1007/s11783-022-1567-y

摘要:

● Evaluated three methods for determining the consortia’s growth kinetics.

关键词: Biodegradation     1     4-Dioxane     Kinetics     Microbial consortium     16S rRNA    

Diversity and distribution of proteorhodopsin-containing microorganisms in marine environments

Bo WEI

《环境科学与工程前沿(英文)》 2012年 第6卷 第1期   页码 98-106 doi: 10.1007/s11783-010-0278-y

摘要: Proteorhodopsin (PR) is a recently discovered protein involved in the utilization of light energy. Several studies have shown that PR-containing microorganisms are widespread and compose a large proportion of the biomass in marine ecosystems. A better understanding of the ecological role of PR will help clarify the effect of the global flow of energy and the carbon cycle on marine communities. In this study, a bioinformatical database of PR codon sequences, the Global Distribution Database of Proteorhodopsin (GDDP), as a tool for analyzing the diversity and distribution of PR-containing microorganisms in marine environments throughout the world was designed. The community structure of PR microorganisms were also compared using PCR assays and UniFrac analyses of 12 samples collected from three water layers (0, 75, and 200 m) at four representative sites in the Pacific, Atlantic, and Indian Oceans. The results indicate that PR-containing microorganisms can be grouped into two distribution types: widespread and location-specific. Representative cases of the former include SAR11-PR and HOT2C01-PR. Interestingly, PR communities cluster by geographic locale but not by water depth.

关键词: proteorhodopsin (PR)     microorganism community     diversity     marine environments    

Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

《环境科学与工程前沿(英文)》 2021年 第15卷 第6期 doi: 10.1007/s11783-021-1426-2

摘要:

• AOA and comammox bacteria can be more abundant and active than AOB/NOB at WWTPs.

关键词: Ammonia oxidizing archaea (AOA)     Complete ammonia oxidizing (comammox) bacteria     Dissimilatory nitrate reduction to ammonium (DNRA) bacteria     Nitrate/nitrite-dependent anaerobic methane oxidizing (NOx-DAMO) microorganisms     Engineering application    

Comparison of growth and lipid accumulation properties of two oleaginous microalgae under different nutrient

Qiao ZHANG,Yu HONG

《环境科学与工程前沿(英文)》 2014年 第8卷 第5期   页码 703-709 doi: 10.1007/s11783-014-0649-x

摘要: This study compared the growth and lipid accumulation properties of two oleaginous microalgae, namely, sp. LX1 and sp. HQ, under different nutrient conditions. Both algal species obtained the highest biomass, lipid content and lipid yield under low-nutrient conditions (mBG11 medium). The biomass, lipid content and lipid yield of sp. LX1 were 0.42 g·L , 22.5% and 93.8 mg·L , respectively. These values were relatively higher than those of sp. HQ (0.30 g·L , 17.1% and 51.3 mg·L , respectively). These algae were then cultivated in an SE medium that contained more nutrients; as a result, the biomass and lipid yield of sp. LX1 reduced more significantly than those of sp. HQ. Opposite results were observed in lipid and triacylglycerols (TAGs) contents. The cell sizes of both algal species under low-nutrient conditions were larger than those under high-nutrient conditions. sp. HQ cells did not aggregate, but sp. LX1 cells flocculated easily, particularly under low-nutrient conditions. In summary, low-nutrient conditions favour the growth and lipid production of both algae, but sp. LX1 outperforms sp. HQ.

关键词: Scenedesmus sp. LX1     Chlorella sp. HQ     growth rate     algal biomass     lipid accumulation     triacylglycerols (TAGs)    

The greater roles of indigenous microorganisms in removing nitrobenzene from sediment compared with the

Xiangqun Chi, Yingying Zhang, Daosheng Wang, Feihua Wang, Wei Liang

《环境科学与工程前沿(英文)》 2018年 第12卷 第1期 doi: 10.1007/s11783-018-1016-0

摘要: The feasibility of using -JS45 system in removing nitrobenzene from sediments was conducted. However, it was observed that nitrobenzene degraded rapidly and was removed completely within 20 days in native sediments, raising the possibility that indigenous microorganisms may play important roles in nitrobenzene degradation. Consequently, this study aimed to verify this possibility and investigate the potential nitrobenzene degraders among indigenous microorganisms in sediments. The abundance of inoculated strain JS45 and indigenous bacteria in sediments was quantified using real-time polymerase chain reaction. Furthermore, community structure of the indigenous bacteria was analyzed through high throughput sequencing based on Illumina MiSeq platform. The results showed that indigenous bacteria in native sediments were abundant, approximately 10 CFU/g dry weight, which is about six orders of magnitude higher than that in fertile soils. In addition, the levels of indigenous ( , , , and ) and ( , , , , and ) increased significantly during nitrobenzene removal. Their quantities sharply decreased after nitrobenzene was removed completely, except for and . Based on the results, it can be concluded that indigenous microorganisms including and can have great potential for removing nitrobenzene from sediments. Although . - JS45 system was set up in an attempt to eliminate nitrobenzene from sediments, and the system did not meet the expectation. The findings still provide valuable information on enhancing nitrobenzene removal by optimizing the sediment conditions for better growth of indigenous and .

关键词: Community structure     Indigenous microorganisms     Nitrobenzene     Plant-microbe associated remediation     Sediment    

Unveiling the interaction mechanisms of key functional microorganisms in the partial denitrification-anammox

《环境科学与工程前沿(英文)》 2023年 第17卷 第8期 doi: 10.1007/s11783-023-1703-3

摘要:

● The availability of PD-anammox was investigated with higher NO3–N concentration.

关键词: PD-anammox process     Nitrite accumulation     COD     Microbial interaction    

Microbial biodegradation of plastics: Challenges, opportunities, and a critical perspective

《环境科学与工程前沿(英文)》 2022年 第16卷 第12期 doi: 10.1007/s11783-022-1596-6

摘要:

● Health hazards of plastic waste on environment are discussed.

关键词: Plastic-waste     Polymers     Health-hazards     Biodegradation     Microorganisms     Enzymes    

ROOT EXUDATES FROM CANOLA EXHIBIT BIOLOGICAL NITRIFICATION INHIBITION AND ARE EFFECTIVE IN INHIBITING AMMONIA OXIDATION IN SOIL

《农业科学与工程前沿(英文)》 2022年 第9卷 第2期   页码 177-186 doi: 10.15302/J-FASE-2021421

摘要:

A range of plant species produce root exudates that inhibit ammonia-oxidizing microorganisms. This biological nitrification inhibition (BNI) capacity can decrease N loss and increase N uptake from the rhizosphere. This study sought evidence for the existence and magnitude of BNI capacity in canola ( Brassica napus). Seedlings of three canola cultivars, Brachiaria humidicola(BNI positive) and wheat ( Triticum aestivum) were grown in a hydroponic system. Root exudates were collected and their inhibition of the ammonia oxidizing bacterium, Nitrosospira multiformis, was tested. Subsequent pot experiments were used to test the inhibition of native nitrifying communities in soil. Root exudates from canola significantly reduced nitrification rates of both N. multiformis cultures and native soil microbial communities. The level of nitrification inhibition across the three cultivars was similar to the well-studied high-BNI species B. humidicola. BNI capacity of canola may have implications for the N dynamics in farming systems and the N uptake efficiency of crops in rotational farming systems. By reducing nitrification rates canola crops may decrease N losses, increase plant N uptake and encourage microbial N immobilization and subsequently increase the pool of organic N that is available for mineralization during the following cereal crops.

关键词: ammonia oxidizing microorganisms / biological nitrification inhibition / farming rotations / nitrogen cycling / nitrogen use efficiency    

Ecotoxicological effect of zinc oxide nanoparticles on soil microorganisms

Zhaoyi SHEN,Zhuo CHEN,Zhen HOU,Tingting LI,Xiaoxia LU

《环境科学与工程前沿(英文)》 2015年 第9卷 第5期   页码 912-918 doi: 10.1007/s11783-015-0789-7

摘要: The widespread production and use of zinc oxide nanoparticles (ZnO-NPs) in recent years have posed potential threat to the ecosystem. This study aimed to investigate the ecotoxicological effect of ZnO-NPs on soil microorganisms using laboratory microcosm test. Respiration, ammonification, dehydrogenase (DH) activity, and fluorescent diacetate hydrolase (FDAH) activity were used as ecotoxicological parameters. The results showed that in the neutral soil treated with 1 mg ZnO-NPs per g soil (fresh, neutral), ammonification was significantly inhibited during the study period of three months, but the inhibition rate decreased over increasing time. Inhibition in respiration was observed in the first month of the test. In various ZnO-NPs treatments (1 mg, 5 mg, and 10 mg ZnO-NPs per g soil), DH activity and FDAH activity were inhibited during the study period of one month. For both enzyme activities, there were positive dose–response relationships between the concentration of ZnO-NPs and the inhibition rates, but the curves changed over time due to changes of ZnO-NPs toxicity. Soil type affected the toxicity of ZnO-NPs in soil. The toxicity was highest in the acid soil, followed by the neutral soil. The toxicity was relatively low in the alkaline soil. The toxicity was not accounted for by the Zn released from the ZnO-NPs. Direct interaction of ZnO-NPs with biologic targets might be one of the reasons. The adverse effect of ZnO-NPs on soil microorganisms in neutral and acid soils is worthy of attention.

关键词: zinc oxide nanoparticles (ZnO-NPs)     soil microorganisms     respiration     ammonification     dehydrogenase (DH) activity     fluorescent diacetate hydrolase (FDAH) activity    

标题 作者 时间 类型 操作

Technologies for extracting lipids from oleaginous microorganisms for biodiesel production

Cunwen WANG, Lu CHEN, Bajpai RAKESH, Yuanhang QIN, Renliang LV

期刊论文

Response of soil microorganisms to vegetational succession in Ziwuling Forest

Hong ZHANG, Jialong LU, Shiwei ZHAO,

期刊论文

Degradation of bisphenol A by microorganisms immobilized on polyvinyl alcohol microspheres

Xue BAI, Hanchang SHI, Zhengfang YE, Qiujin SUN, Qing WANG, Zhongyou WANG

期刊论文

Advances in airborne microorganisms detection using biosensors: A critical review

期刊论文

Formation of disinfection byproducts from accumulated soluble products of oleaginous microalga after

Yu Liu, Qiao Zhang, Yu Hong

期刊论文

Inactivation and risk control of pathogenic microorganisms in municipal sludge treatment: A review

期刊论文

Determination of growth kinetics of microorganisms linked with 1,4-dioxane degradation in a consortium

期刊论文

Diversity and distribution of proteorhodopsin-containing microorganisms in marine environments

Bo WEI

期刊论文

Overlooked nitrogen-cycling microorganisms in biological wastewater treatment

期刊论文

Comparison of growth and lipid accumulation properties of two oleaginous microalgae under different nutrient

Qiao ZHANG,Yu HONG

期刊论文

The greater roles of indigenous microorganisms in removing nitrobenzene from sediment compared with the

Xiangqun Chi, Yingying Zhang, Daosheng Wang, Feihua Wang, Wei Liang

期刊论文

Unveiling the interaction mechanisms of key functional microorganisms in the partial denitrification-anammox

期刊论文

Microbial biodegradation of plastics: Challenges, opportunities, and a critical perspective

期刊论文

ROOT EXUDATES FROM CANOLA EXHIBIT BIOLOGICAL NITRIFICATION INHIBITION AND ARE EFFECTIVE IN INHIBITING AMMONIA OXIDATION IN SOIL

期刊论文

Ecotoxicological effect of zinc oxide nanoparticles on soil microorganisms

Zhaoyi SHEN,Zhuo CHEN,Zhen HOU,Tingting LI,Xiaoxia LU

期刊论文